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Procell Inc complete culture medium for huvec
AEP inhibitor 7,8‐DHF <t>protect</t> <t>HUVECs</t> against oxygen–glucose deprivation by inhibiting tPA‐induced elevated LRP‐1, MMP2, and MMP9. HUVECs treated with 0.5 μM 7,8‐DHF for 24 h followed by OGD 4 h and reoxygen‐glucose plus tPA (500 ng/mL) for another 24 h as the methods part described. (A, B) Western blotting to evaluate the expression of ZO‐1, claudin5, occluding, and JAM‐1. (C) The viability of HUVECs at different time points. (D) AEP enzymatic analysis of HUVECs subjected to 24 h tPA treatment following 7,8‐DHF and OGD. (E, F) Western blotting to evaluate the expression of AEP, p‐TrkB, TrkB, LRP‐1, MMP2, and MMP9. (A) n = 4, (C) n = 6, (D) n = 4, (E) n = 4 per group. Data are presented as mean ± SEM, and statistical analysis is performed using one‐way ANOVA test followed by Tukey's multiple comparisons test when P value of Levene test > 0.05 or Welch test followed by Dunnett T3 multiple comparisons test when the P value of Levene test < 0.05. Normality and variance are assessed via Shapiro‐Wilk test and Levene's test, respectively. * P < 0.05, ** P < 0.01, *** P < 0.001.
Complete Culture Medium For Huvec, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/complete culture medium for huvec/product/Procell Inc
Average 90 stars, based on 1 article reviews
complete culture medium for huvec - by Bioz Stars, 2026-03
90/100 stars

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1) Product Images from "Asparagine Endopeptidase Inhibition Attenuates Tissue Plasminogen Activator‐Induced Brain Hemorrhagic Transformation After Ischemic Stroke"

Article Title: Asparagine Endopeptidase Inhibition Attenuates Tissue Plasminogen Activator‐Induced Brain Hemorrhagic Transformation After Ischemic Stroke

Journal: CNS Neuroscience & Therapeutics

doi: 10.1111/cns.70345

AEP inhibitor 7,8‐DHF protect HUVECs against oxygen–glucose deprivation by inhibiting tPA‐induced elevated LRP‐1, MMP2, and MMP9. HUVECs treated with 0.5 μM 7,8‐DHF for 24 h followed by OGD 4 h and reoxygen‐glucose plus tPA (500 ng/mL) for another 24 h as the methods part described. (A, B) Western blotting to evaluate the expression of ZO‐1, claudin5, occluding, and JAM‐1. (C) The viability of HUVECs at different time points. (D) AEP enzymatic analysis of HUVECs subjected to 24 h tPA treatment following 7,8‐DHF and OGD. (E, F) Western blotting to evaluate the expression of AEP, p‐TrkB, TrkB, LRP‐1, MMP2, and MMP9. (A) n = 4, (C) n = 6, (D) n = 4, (E) n = 4 per group. Data are presented as mean ± SEM, and statistical analysis is performed using one‐way ANOVA test followed by Tukey's multiple comparisons test when P value of Levene test > 0.05 or Welch test followed by Dunnett T3 multiple comparisons test when the P value of Levene test < 0.05. Normality and variance are assessed via Shapiro‐Wilk test and Levene's test, respectively. * P < 0.05, ** P < 0.01, *** P < 0.001.
Figure Legend Snippet: AEP inhibitor 7,8‐DHF protect HUVECs against oxygen–glucose deprivation by inhibiting tPA‐induced elevated LRP‐1, MMP2, and MMP9. HUVECs treated with 0.5 μM 7,8‐DHF for 24 h followed by OGD 4 h and reoxygen‐glucose plus tPA (500 ng/mL) for another 24 h as the methods part described. (A, B) Western blotting to evaluate the expression of ZO‐1, claudin5, occluding, and JAM‐1. (C) The viability of HUVECs at different time points. (D) AEP enzymatic analysis of HUVECs subjected to 24 h tPA treatment following 7,8‐DHF and OGD. (E, F) Western blotting to evaluate the expression of AEP, p‐TrkB, TrkB, LRP‐1, MMP2, and MMP9. (A) n = 4, (C) n = 6, (D) n = 4, (E) n = 4 per group. Data are presented as mean ± SEM, and statistical analysis is performed using one‐way ANOVA test followed by Tukey's multiple comparisons test when P value of Levene test > 0.05 or Welch test followed by Dunnett T3 multiple comparisons test when the P value of Levene test < 0.05. Normality and variance are assessed via Shapiro‐Wilk test and Levene's test, respectively. * P < 0.05, ** P < 0.01, *** P < 0.001.

Techniques Used: Western Blot, Expressing



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Procell Inc complete culture medium for huvec
AEP inhibitor 7,8‐DHF <t>protect</t> <t>HUVECs</t> against oxygen–glucose deprivation by inhibiting tPA‐induced elevated LRP‐1, MMP2, and MMP9. HUVECs treated with 0.5 μM 7,8‐DHF for 24 h followed by OGD 4 h and reoxygen‐glucose plus tPA (500 ng/mL) for another 24 h as the methods part described. (A, B) Western blotting to evaluate the expression of ZO‐1, claudin5, occluding, and JAM‐1. (C) The viability of HUVECs at different time points. (D) AEP enzymatic analysis of HUVECs subjected to 24 h tPA treatment following 7,8‐DHF and OGD. (E, F) Western blotting to evaluate the expression of AEP, p‐TrkB, TrkB, LRP‐1, MMP2, and MMP9. (A) n = 4, (C) n = 6, (D) n = 4, (E) n = 4 per group. Data are presented as mean ± SEM, and statistical analysis is performed using one‐way ANOVA test followed by Tukey's multiple comparisons test when P value of Levene test > 0.05 or Welch test followed by Dunnett T3 multiple comparisons test when the P value of Levene test < 0.05. Normality and variance are assessed via Shapiro‐Wilk test and Levene's test, respectively. * P < 0.05, ** P < 0.01, *** P < 0.001.
Complete Culture Medium For Huvec, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/complete culture medium for huvec/product/Procell Inc
Average 90 stars, based on 1 article reviews
complete culture medium for huvec - by Bioz Stars, 2026-03
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Procell Inc complete culture medium for pumc-huvec-t1 cm-0675
AEP inhibitor 7,8‐DHF <t>protect</t> <t>HUVECs</t> against oxygen–glucose deprivation by inhibiting tPA‐induced elevated LRP‐1, MMP2, and MMP9. HUVECs treated with 0.5 μM 7,8‐DHF for 24 h followed by OGD 4 h and reoxygen‐glucose plus tPA (500 ng/mL) for another 24 h as the methods part described. (A, B) Western blotting to evaluate the expression of ZO‐1, claudin5, occluding, and JAM‐1. (C) The viability of HUVECs at different time points. (D) AEP enzymatic analysis of HUVECs subjected to 24 h tPA treatment following 7,8‐DHF and OGD. (E, F) Western blotting to evaluate the expression of AEP, p‐TrkB, TrkB, LRP‐1, MMP2, and MMP9. (A) n = 4, (C) n = 6, (D) n = 4, (E) n = 4 per group. Data are presented as mean ± SEM, and statistical analysis is performed using one‐way ANOVA test followed by Tukey's multiple comparisons test when P value of Levene test > 0.05 or Welch test followed by Dunnett T3 multiple comparisons test when the P value of Levene test < 0.05. Normality and variance are assessed via Shapiro‐Wilk test and Levene's test, respectively. * P < 0.05, ** P < 0.01, *** P < 0.001.
Complete Culture Medium For Pumc Huvec T1 Cm 0675, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/complete culture medium for pumc-huvec-t1 cm-0675/product/Procell Inc
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AllCells LLC huvec complete culture medium
AEP inhibitor 7,8‐DHF <t>protect</t> <t>HUVECs</t> against oxygen–glucose deprivation by inhibiting tPA‐induced elevated LRP‐1, MMP2, and MMP9. HUVECs treated with 0.5 μM 7,8‐DHF for 24 h followed by OGD 4 h and reoxygen‐glucose plus tPA (500 ng/mL) for another 24 h as the methods part described. (A, B) Western blotting to evaluate the expression of ZO‐1, claudin5, occluding, and JAM‐1. (C) The viability of HUVECs at different time points. (D) AEP enzymatic analysis of HUVECs subjected to 24 h tPA treatment following 7,8‐DHF and OGD. (E, F) Western blotting to evaluate the expression of AEP, p‐TrkB, TrkB, LRP‐1, MMP2, and MMP9. (A) n = 4, (C) n = 6, (D) n = 4, (E) n = 4 per group. Data are presented as mean ± SEM, and statistical analysis is performed using one‐way ANOVA test followed by Tukey's multiple comparisons test when P value of Levene test > 0.05 or Welch test followed by Dunnett T3 multiple comparisons test when the P value of Levene test < 0.05. Normality and variance are assessed via Shapiro‐Wilk test and Levene's test, respectively. * P < 0.05, ** P < 0.01, *** P < 0.001.
Huvec Complete Culture Medium, supplied by AllCells LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/huvec complete culture medium/product/AllCells LLC
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Lonza complete huvec culture medium egm-2 with bulletkit
AEP inhibitor 7,8‐DHF <t>protect</t> <t>HUVECs</t> against oxygen–glucose deprivation by inhibiting tPA‐induced elevated LRP‐1, MMP2, and MMP9. HUVECs treated with 0.5 μM 7,8‐DHF for 24 h followed by OGD 4 h and reoxygen‐glucose plus tPA (500 ng/mL) for another 24 h as the methods part described. (A, B) Western blotting to evaluate the expression of ZO‐1, claudin5, occluding, and JAM‐1. (C) The viability of HUVECs at different time points. (D) AEP enzymatic analysis of HUVECs subjected to 24 h tPA treatment following 7,8‐DHF and OGD. (E, F) Western blotting to evaluate the expression of AEP, p‐TrkB, TrkB, LRP‐1, MMP2, and MMP9. (A) n = 4, (C) n = 6, (D) n = 4, (E) n = 4 per group. Data are presented as mean ± SEM, and statistical analysis is performed using one‐way ANOVA test followed by Tukey's multiple comparisons test when P value of Levene test > 0.05 or Welch test followed by Dunnett T3 multiple comparisons test when the P value of Levene test < 0.05. Normality and variance are assessed via Shapiro‐Wilk test and Levene's test, respectively. * P < 0.05, ** P < 0.01, *** P < 0.001.
Complete Huvec Culture Medium Egm 2 With Bulletkit, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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AEP inhibitor 7,8‐DHF protect HUVECs against oxygen–glucose deprivation by inhibiting tPA‐induced elevated LRP‐1, MMP2, and MMP9. HUVECs treated with 0.5 μM 7,8‐DHF for 24 h followed by OGD 4 h and reoxygen‐glucose plus tPA (500 ng/mL) for another 24 h as the methods part described. (A, B) Western blotting to evaluate the expression of ZO‐1, claudin5, occluding, and JAM‐1. (C) The viability of HUVECs at different time points. (D) AEP enzymatic analysis of HUVECs subjected to 24 h tPA treatment following 7,8‐DHF and OGD. (E, F) Western blotting to evaluate the expression of AEP, p‐TrkB, TrkB, LRP‐1, MMP2, and MMP9. (A) n = 4, (C) n = 6, (D) n = 4, (E) n = 4 per group. Data are presented as mean ± SEM, and statistical analysis is performed using one‐way ANOVA test followed by Tukey's multiple comparisons test when P value of Levene test > 0.05 or Welch test followed by Dunnett T3 multiple comparisons test when the P value of Levene test < 0.05. Normality and variance are assessed via Shapiro‐Wilk test and Levene's test, respectively. * P < 0.05, ** P < 0.01, *** P < 0.001.

Journal: CNS Neuroscience & Therapeutics

Article Title: Asparagine Endopeptidase Inhibition Attenuates Tissue Plasminogen Activator‐Induced Brain Hemorrhagic Transformation After Ischemic Stroke

doi: 10.1111/cns.70345

Figure Lengend Snippet: AEP inhibitor 7,8‐DHF protect HUVECs against oxygen–glucose deprivation by inhibiting tPA‐induced elevated LRP‐1, MMP2, and MMP9. HUVECs treated with 0.5 μM 7,8‐DHF for 24 h followed by OGD 4 h and reoxygen‐glucose plus tPA (500 ng/mL) for another 24 h as the methods part described. (A, B) Western blotting to evaluate the expression of ZO‐1, claudin5, occluding, and JAM‐1. (C) The viability of HUVECs at different time points. (D) AEP enzymatic analysis of HUVECs subjected to 24 h tPA treatment following 7,8‐DHF and OGD. (E, F) Western blotting to evaluate the expression of AEP, p‐TrkB, TrkB, LRP‐1, MMP2, and MMP9. (A) n = 4, (C) n = 6, (D) n = 4, (E) n = 4 per group. Data are presented as mean ± SEM, and statistical analysis is performed using one‐way ANOVA test followed by Tukey's multiple comparisons test when P value of Levene test > 0.05 or Welch test followed by Dunnett T3 multiple comparisons test when the P value of Levene test < 0.05. Normality and variance are assessed via Shapiro‐Wilk test and Levene's test, respectively. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The HUVECs were cultured in complete culture medium for HUVEC (CM‐0122, Procell Life Science & Technology, China).

Techniques: Western Blot, Expressing